How to Reconstitute BPC-157: Step-by-Step Dilution, Math & Storage Guide
5mg Vial + 2mL BAC Water = 2.5 mg/mL (250 mcg per 10 Units on a U-100 syringe)
Standard Diluent: 0.9% Benzyl Alcohol Bacteriostatic Water (USP)
Required Laboratory Equipment
- 5mg BPC-157 Lyophilized Vial
- Bacteriostatic Water (USP Grade) — Multi-dose sterile preservative diluent
- Alcohol prep pads
- 3mL Syringe + 23G needle (mixing)
- U-100 0.5mL / 1.0mL 30G Insulin Syringes
Step-by-Step Reconstitution Protocol
- Wipe down the rubber tops of both vials using sterile alcohol prep pads and let dry.
- Using a 3mL syringe, draw 2.0 mL of Bacteriostatic Water.
- Angle the needle against the inner glass wall of the BPC-157 vial and inject slowly so the diluent cascades gently down the glass.
- Swirl the vial gently until the white powder dissolves completely. BPC-157 dissolves rapidly within 10 to 30 seconds.
- Affix a label with the reconstitution date and exact concentration (2.5mg/mL).
- Keep refrigerated at 2°C - 8°C. Stable for up to 45 days in refrigerated storage.
Syringe Draw Volume by Target Dose
| Target Dose (mcg) | U-100 Syringe Draw | U-50 Syringe Draw |
|---|---|---|
| 250 mcg | 10 Units (0.1 mL) | 5 Units |
| 500 mcg | 20 Units (0.2 mL) | 10 Units |
| 750 mcg | 30 Units (0.3 mL) | 15 Units |
Safety & Sterility Guidelines (USP <797>)
- Do not use plain sterile water (without benzyl alcohol) if multi-dose use extends past 24 hours, as bacterial contamination will occur.
- Store in a dedicated dark container inside the refrigerator away from door vibrations.