How to Reconstitute Retatrutide: Step-by-Step BAC Water & Syringe Math Guide

10mg Vial + 2mL BAC Water = 5.0 mg/mL (50 mcg per 1 Syringe Unit on U-100)
Standard Diluent: 0.9% Benzyl Alcohol Bacteriostatic Water (USP)

Required Laboratory Equipment

  • Retatrutide Lyophilized Powder Vial (5mg, 10mg, or 15mg)
  • Bacteriostatic Water (0.9% Benzyl Alcohol) — Multi-dose sterile preservative diluent
  • 70% Isopropyl Alcohol Prep Pads
  • 3mL Luer-Lock Syringe with 21G - 25G Needle (for reconstitution)
  • U-100 31G Insulin Syringes (for research dosing)

Step-by-Step Reconstitution Protocol

  1. Disinfect the rubber stoppers of both the Bacteriostatic Water vial and the Retatrutide lyophilized peptide vial with fresh 70% isopropyl alcohol swabs. Allow to air dry for 30 seconds.
  2. Draw exactly 2.0 mL of air into a 3mL mixing syringe. Inject the air into the BAC water vial and withdraw 2.0 mL of bacteriostatic water.
  3. Insert the mixing needle into the Retatrutide vial at a 45-degree angle against the inner glass wall. Release the water slowly down the side of the glass. NEVER squirt liquid directly onto the lyophilized powder cake.
  4. Allow the vacuum inside the vial to pull the plunger naturally if present. Remove the needle once liquid transfer is complete.
  5. Gently roll the vial between your palms for 60-90 seconds. DO NOT shake or vortex the vial, as peptide chains are delicate and can shear.
  6. Inspect the reconstituted solution: it should be crystal clear with zero floating particulate matter.
  7. Store immediately in the refrigerator between 2°C - 8°C (36°F - 46°F). Protect from direct light.

Syringe Draw Volume by Target Dose

Target Dose (mcg) U-100 Syringe Draw U-50 Syringe Draw
1000 mcg 20 Units (0.2 mL) 10 Units
2000 mcg 40 Units (0.4 mL) 20 Units
4000 mcg 80 Units (0.8 mL) 40 Units

Safety & Sterility Guidelines (USP <797>)

  • Never reuse needles or mixing syringes.
  • Always check that the BAC water is clear and not past its 28-day puncture limit.
  • Do not freeze reconstituted Retatrutide; freeze-thaw cycles destroy peptide quaternary structure.
View RETATRUTIDE Clinical Dossier → 💧 Master BAC Water Guide → ⚡ Interactive Syringe Calculator →