How to Reconstitute Semaglutide: Dilution Math & Syringe Tick Guide
5mg Vial + 2mL BAC Water = 2.5 mg/mL (250 mcg per 10 Units on U-100)
Standard Diluent: 0.9% Benzyl Alcohol Bacteriostatic Water (USP)
Required Laboratory Equipment
- Semaglutide 5mg vial
- BAC water
- Alcohol pads
- U-100 syringes
Step-by-Step Reconstitution Protocol
- Swab rubber tops with alcohol.
- Inject 2.0 mL BAC water down the glass wall.
- Swirl gently until completely transparent.
- Keep refrigerated between 2°C and 8°C.
Syringe Draw Volume by Target Dose
| Target Dose (mcg) | U-100 Syringe Draw | U-50 Syringe Draw |
|---|---|---|
| 250 mcg | 10 Units (0.1 mL) | 5 Units |
| 500 mcg | 20 Units (0.2 mL) | 10 Units |
| 1000 mcg | 40 Units (0.4 mL) | 20 Units |
Safety & Sterility Guidelines (USP <797>)
- Solution must be fully clear and colorless before use.