How to Reconstitute TB-500 (Thymosin Beta-4): Mixing & Reconstitution Math
5mg Vial + 2mL BAC Water = 2.5 mg/mL (2500 mcg per 1.0 mL)
Standard Diluent: 0.9% Benzyl Alcohol Bacteriostatic Water (USP)
Required Laboratory Equipment
- TB-500 5mg or 10mg vial
- Bacteriostatic water — Multi-dose sterile preservative diluent
- Alcohol swabs
- U-100 1mL Insulin syringes
Step-by-Step Reconstitution Protocol
- Clean rubber stoppers thoroughly with 70% alcohol pads.
- Draw 2.0 mL of Bacteriostatic Water using a 3mL syringe.
- Slowly stream the water against the vial glass wall to avoid foaming.
- Swirl gently until completely clear.
- Store at 2°C - 8°C in a dark refrigerator.
Syringe Draw Volume by Target Dose
| Target Dose (mcg) | U-100 Syringe Draw | U-50 Syringe Draw |
|---|---|---|
| 1000 mcg | 40 Units (0.4 mL) | 20 Units |
| 2000 mcg | 80 Units (0.8 mL) | 40 Units |
| 2500 mcg | 100 Units (1 mL) | 50 Units |
Safety & Sterility Guidelines (USP <797>)
- TB-500 can be combined in the same syringe with BPC-157 immediately before administration for the Wolverine Stack protocol.